Lab Chronicles: Crystalized vaxx with Raman Spec

Raman Spectroscopy is a non-destructive chemical analysis technique which provides detailed information about chemical structure, phase and polymorphy, crystallinity and molecular interactions. It is based upon the interaction of light with the chemical bonds within a material.  Dr McCairn has samples that have crystalized making them suitable for Raman spec.

This is a short stream where Dr McCairn is again looking for a phosphorus signature to see if any RNA is present in the sample. Previously he used SEM/EDX and now Raman Spectroscopy. Raman can also be used for Quantitative label-free RNA detection. At one point Kevin holds up this article (reversed by the camera) to his audience.  This shows that pure samples at known concentrations of RNA/DNA can be detected using Raman.

https://t.co/qsaVwkp815

— The Tishbite (@Tishbite_redux) June 30, 2022

The crystal give the same results using Raman as those using the  EDX/SEM analytical techniques namely an absence of detectable phosphorus (P) in both Moderna and Pfizer suggesting that the samples only contain lipids (Cholesterol/PEG).  The typical double graphene peak at 1500nm was absent putting to rest the speculation about graphene. All the peaks are about 1100 nm and indicative of the Carbon-Hydrogen bonds formed by lipids. There is no phosphorus (P) present.  I have screen shot and gone over the trace on Kevin’s computer screen with red.  Hopefully Kevin will produce a fuller report when all his experiments are completed.

2./ pic.twitter.com/srz8rxcZSI

— The Tishbite (@Tishbite_redux) June 30, 2022

4./ pic.twitter.com/D7rLLW53dx

— The Tishbite (@Tishbite_redux) June 30, 2022

6./ PEG traces depended on the length of the chain, the temperature and any cross-linking. Some of these are very long polymers. There may be a mixture of different chain lengths. https://t.co/DGdV0X4WVi pic.twitter.com/WbLsXbNtDT

— The Tishbite (@Tishbite_redux) June 30, 2022

Dr McCairn is trying to conserve lab-time because it is expensive but from half way the month he will gain access to multiple fresh production vaccine samples and will not have to wait for customs clearance. He is also looking to acquire pure DNA/RNA and lipid standardized samples as controls.  So far there is no phosphorus signature in the Moderna or Pfizer samples which is unusual because phosphorus forms part of the sugar back-bone of all RNA and DNA biological samples.  They look like non-functional samples (placebos). Dr McCairn wants to try NRM, PCR and especially cell cultures to see if he can induce amyloid formation.

On WTYL:

Lab Chronicles:Looking at Crystalized Moderna with Raman Spectroscopy (37 min)

Here is the Odysee version:

( to be posted when ready)