Tag: 3′-UTR

Stop Condons

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Stop Condons

It is becoming clear that enormous risks have been taken with this experimental procedure which is the precursor platform to gene therapy.  The Fourth Industrial Revolution is riding on this new technology and they have pushed ahead despite all the unknowns. In order to make it work they had to stabilize the mRNA otherwise it would be degraded by the body. They did this by methylating the uridine to pseudouridine.

However, this can cause other problems such as reading through “stop condons”.  If you have no idea what I am talking about I suggest you look at this previous article and specifically watch the video at the end with Dr Kevin McKernan and Dr. Jay Couey.

Sherlock Ohmes

 

A cell replicates itself using its genomic intels. To do this, it reproduces what is available in DNA, until the stop codon (red) is met. The orange area is the most important part to get a perfect clone that does exactly what it is intended to do but, there is a way for the 3′-UTR (purple) to be included in the duplicated cell, which produces a cell that does something else. Something unknown.

3′-UTR is the Three prime untranslated region . In molecular genetics, the three prime untranslated region (3′-UTR) is the section of messenger RNA (mRNA) that immediately follows the translation termination codon. The 3′-UTR often contains regulatory regions that post-transcriptionally influence gene expression.

Putting on the brakes

In order to prevent this happening they have put more than one stop condon in.

Why use two stop UGA codons instead of one in the spike protein mRNA for the BioNTech/Pfizer SARS-CoV-2 vaccine?

 

Read Through

People have valid concerns that “read through ” will occur on the stop condons and it is known that in certain circumstances antibiotics can cause this. Here are some comments discussing the problem.

RefSeq curation and annotation of stop codon recoding in vertebrates

So it’s the alteration from uridine to pseudouridine allowing the codons to be read in the ribosome to instruct it to make the artificial S protein, but in the very act of that alteration, it screws up the “genetic compiler”, thereby misreading the code and translating the 3’UTR? There is a good chance that at least some (how many?) will readthrough causing the 3_UTR to be translated and attached to Spike Protein. And then that could cause Spike to mis-fold somehow (on this see McKernan). But NO testing was ever done to see what happens. They didn’t want to know.

If they already had their perfect 5’UTR in 2019 they wouldn’t have had to test 280,000 new ones, but what do I know eh?

OAS and ADE

Original Antigenic Sin and Antibody Dependent Enhancement.

 

The “original antigenic sin” and its relevance for SARS-CoV-2 (COVID-19) vaccination

Conclusion

What would I know?   I am not a geneticist just a bucket chemist. However, I know enough to hear alarm bells sounding. Especially when pharma hides data.  Especially when they have indemnity.

Biohazard Alarm[WARNING: The sound is very LOUD] 49secs