Gigaohm Biological (July 28)

Gigaohm Biological (July 28)

Phoshpholipid

Another excellent presentation with Walter Chestnut discussing his latest findings, also commenting on a Robert Malone video who warns about the LNP delivery mechanism causing problems such as PEG micelles stimulating production of anti-PEG IgM, which led to accelerated clearance of subsequently administered PEGylated liposomes. According to A review on phospholipids and their main applications in drug delivery systems, Phoshpholipid–PE–PEG mixtures could form micelles rather than liposomes if PE–PEG content exceeds certain critical limit.  Of course the PEG  interests me because it is used along with surfactants for micelle formation in polymerisations.  I never thought something like that would be injected into people (lolz). Below is actually a good resource on Lipids (have a look at all the pages very informative):

 

Heavy metals play a role in neurodegeneration  but lipids are important as  Anticardiolipin and other antiphospholipid antibodies (are found) in critically ill COVID-19 positive and negative patients Antiphospholipid antibodies (APLAs) are biomarkers of a spectrum of clinical features observed in antiphospholipid syndrome (APS).1 Features of APS include venous and arterial thrombosis involving multiple organs and having various presentations. Positive APLA serology was associated with more severe disease regardless of COVID-19 status.  Moreover, Persistent lgG anticardiolipin autoantibodies are associated with post-COVID syndromePersistence of various symptoms in patients who have recovered from coronavirus disease 2019 (COVID-1 9) was recently defined as ‘long COVID’ or ‘post-COVID syndrome’ (PCS). They conclude that Immunoglobulin G (lgG) anticardiolipin autoantibodies (aCL) are associated with the severity of COVID-19.  In other words an allergic or inflammatory reaction to lipid (in the heart) but also “…persistence of the virus, especially in the nervous system, could be suggested with a post-infectious inflammatory or autoimmune reaction”.  Might this have something to do with pumping the body full of phoshpholipids and mRNA spikes? What do I know? (I am just a bucket chemist who used to mix this stuff in buckets not in human bodies).

Unfortunately, many of Walter’s hypothesis are proving to be correct. He is an avid reader of the medical literature and a talented dot-connecter with a big picture overview. His sub-stack is highly recommended.  In his latest article he shows how amyloid is a double-edged sword. A large build-up will cause problems, but amyloids also serve protective functions – against autoimmune attacks.  So, they are symptomatic rather than causal factors.

Comment: Repeated infection with covids is not good even for the unjabbed and even if they got over it before. A least the omicrons have the PrP silenced. But the HIV and the other nasties and all the humanized nucleic acid etc tacked into the spike yes, will be immune insults that will catch up with us the older we are. Shortened lifetimes, yes. The younger the less shortened. Of course, the jabbed are the ones who will suffer shortest lives.

More study is needed into the mutagenic properties of the spike-protein. Perhaps this explains Nucleic Acid Acid Based Therapeutics against Respiratory Infections?

Comment: The prion promoting sequence in the spike also promotes amyloidosis. They are seeing where there won't be a positive D-dimer test but when they put blood under the scope then you see the amyloid building up. The amyloid is causing the clotting, the heart damage, etc. They look for a test for amyloid in those "rare" pre-jab liver inflammation cases and they find amyloid build up and light chains. The hepatitis victims go on to be found Dx with myloid leukemia (if I have the name correct). The Chinese are making more targeted covid viruses - but it is US research over 25 years that developed those - there is research trail from Baric, Daszak and others and a lot of patents. Baric collaborated with Shi - he trained her! That enrichment is bad news - they did that in the jab for a lot of reasons - it throws off all kinds of cascades along with the defanging IFN-1. IFN-1 has all these cascades which include regulatory ones to down regulate inflammation. But that is all gone because of the jab made to evade being recognized and take IFN-1 out. Enriching with C-G over-glycosylates it possibly more than HIV is so it sails on by more so than the virus construct.

This is very complex and there is so much that we do not know. As someone commented.

Code that codes for multiple things depending happening on how it’s parsed with the folds everybody producing the spikes internally become a mini gain of function study. multiply by a billion consecutive studies running concurrently.

It is not just the structure of the protein but how it folds and polarity as in hydrophilic or hydrophobic nature and charge.  Who is to say that the same protein might not fold differently under other circumstances thereby acquiring different properties?  Repeat expansions have been proposed to cause disease by both the repeat RNA forming foci that sequester RNA-binding proteins and through toxic dipeptide repeat proteins generated by repeat-associated non-ATG translation discussed in the article  G-quadruplex-binding small molecules ameliorate C9orf72 FTD/ALS pathology in vitro and in vivo.  

C9orf72 (chromosome 9 open reading frame 72) is a protein which in humans is encoded by the gene C9orf72. The protein is found in many regions of the brain, in the cytoplasm of neurons as well as in presynaptic terminals. The mutations in C9orf72 are significant because it is the first pathogenic mechanism identified to be a genetic link between familial frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). The mutation of C9ORF72 is a hexanucleotide repeat expansion of the six-letter string of nucleotides GGGGCC.

I wonder, I wonder if pumping the body full of mRNA instructions to make spike proteins will deliver the spike proteins that they want?  We know already that the mRNA vaccines are not pure but contain fragments.   The start codon of a gene is always “ATG” so repeat associated non-ATG translation means that it does not read the correct starting point, or you could say that it reads through the stop condon (runs the red light).  It looks like read-through can cause disease. Is that why the above experiment attempted to improve dementia using small peptides that stick to the G-quadruplex?

Walter Chesnut LIVE: Gigaohm Biological High Resistance Low Noise Information Brief (2:50)

Globo-homo

Globo-homo

A three hour stream in which  WEF’s Juval Harari Globo-homo Wet Dream For Ukraine is contrasted with the Actual Facts On The Ground.

Dojo Time Stamps:

Virus evolution and LNP

Virus evolution and LNP

The Explosion of Cancer and Latent Disease: The Lipid Nanoparticle Platform Is Asking for Trouble (5 min)

Virus evolution by antibodies + BA 2 Omicron – COVID-19 vaccines update 40 (17 min)

How does the virus evolve inside an infected person – we commence this story with a new demonstration in a vaccinated patient, and relapse to a well-documented history in COVID-19 patients, and how antibodies can be contributing to such viral evolution. This also leads us to a hypothesis as to why evolving variants might show up in wastewater prior to taking over a population. We conclude with how the BA2 variant of the Omicron is different from the BA1 variant, demonstrating continuous uninterrupted evolution of the virus.

Described content: Vaccine patient variants: https://www.sciencedirect.com/science… Variants database: https://cov-lineages.org/lineage_list… Innunocompromised host: https://www.nejm.org/doi/10.1056/NEJM… Cancer host: https://linkinghub.elsevier.com/retri… HIV host: https://www.medrxiv.org/content/10.11… Prolonged infection variants: https://academic.oup.com/ve/article/7… Evolution speed: https://www.clinicalmicrobiologyandin… Immune escape co-evolution: https://www.science.org/doi/10.1126/s… Convalescent plasma host: https://www.nature.com/articles/s4158… GIT variant evolution: https://genomemedicine.biomedcentral…. GIT viral load: https://www.gastrojournal.org/article… Viral load impact: https://academic.oup.com/ve/article/7… BA2 Omicron: https://arxiv.org/abs/2202.05031

Gigaohm Biological Breakthrough(20 April)

Gigaohm Biological Breakthrough(20 April)

Highly Recommended

This stream by Jonathan Couey is highly recommend and so is the previous stream by Dr Kevin  McCairn under the Lab Chronicles tab.

Jonathan is on fire and on the verge of a big break-through.  He was contacted by someone important whose, “voice we would recognize”.   I wonder who that could be?  Perhaps the person he tweeted about recently?

Senator Robert F. Kennedy Jr. from Children’s Health Defense. Kennedy is a son of U.S. senator Robert F. Kennedy and a nephew of President John F. Kennedy. He saw them both slaughtered by the Deep State and is called an anti-vaxxer and conspiracy theorist by Wikipedia. No higher recommendation is possible (lolz).

J.C.  was also contacted by Senator Ronald Harold Johnson is an American accountant, businessman, and politician serving as the senior United States senator from Wisconsin. A Republican, Johnson was first elected to the U.S. Senate in 2010. Johnson chaired the Covid Opinion panel with a number of top doctors and scientists.

These are two of the subjects touched upon by Jonathan…..(shocking….shocking….)

Immunomythology Update: Gigaohm Biological High Resistance Low Noise Information Brief  (2:08)

TGA Judicial Review

TGA Judicial Review

I have come across Julian Gillespie before and knew he was perusing a judicial review in Australia and have just been reminded (by my sister) of the progress  he is making.

The TGA is similar to the FDA in the USA. The Therapeutic Goods Administration (TGA) is the medicine and therapeutic regulatory agency of the Australian Government. As part of the Department of Health, the TGA regulates the quality, supply and advertising of medicines, pathology devices, medical devices, blood products and most other therapeutics.

Mr Julian Gillespie is a retired lawyer and former barrister who has come out of retirement to fight the legal battle against the COVID vaccination. He believes that the Australian people have not been given accurate information around COVID deaths, and deaths from the COVID vaccinations.

He is currently managing proceedings in the Federal Court of Australia related to “Australian Vaccination-Risks Network Incorporated v. Secretary, Department of Health.”

COVID UNDER QUESTION is a cross-party inquiry into the Government’s response to COVID held on 23rd March 2022. COVID Under Question was hosted by Senator Malcolm Roberts (One Nation Federal Senator for Queensland) and attended by Stephen Andrew (One Nation Queensland State MP for Mirani), George Christensen (Federal Nationals MP for Dawson), Gerard Rennick (Federal Liberal Senator for Queensland), Alex Antic (Federal Liberal Senator for South Australia) and Craig Kelly (Federal Palmer United Australia MP for Hughes).

Parliamentarians heard from a range of Doctors, experts, economists and everyday people about how the Government’s response to COVID has affected them and at times defied belief. The absurdity of Chief Health Officer dictates and power hungry politicians is all laid bare.

The full day’s proceedings were recorded and are available here: https://www.malcolmrobertsqld.com.au/covid-under-question-a-cross-party-inquiry/

COVID Under Question: Mr Julian Gillespie on March 24  (34 min)

TGA Judicial Review update #4 with Julian Gillespie March 8 (1:01)

Walter M Chestnut

Walter M Chestnut

This video features an interview with the Independent researcher and twitter phenomenon that is Walter Chestnut who is a composer by trade and who has a prodigious capacity to consume research articles and find links thus enabling him to suggest different hypotheses which are documented on his website https://wmcresearch.org/  and tweeted out. His work has already borne fruit as he made Kevin aware of the prion domain and prion genesis. He has however been dismissed by the likes of Kristian Andersen who wrote to Anthony Fauci about the possibility of an engineered coronavirus and then later back-tracked and participated in the cover-up.  As far as these people are concerned if you are not credentialed you should just shut-up and listen to your betters (while they screw you over). However, many of Walter’s theories have proven correct and it is refreshing to have someone with a sharp mind and unhindered by bias and preconception proposing hypotheses.  In case anyone has forgotten that is how science progresses.  It never progresses through consensus.

Kevin and Walter discuss a leaked report by  Acuitas Therapeutics on how testing LNP (Lipid nano-particles) caused damage to rats. Report: “[A Tissue Distribution Study of a [³H]-Labelled Lipid Nanoparticle-mRNA Formulation Containing ALC-0315 and ALC-0159 Following Intramuscular Administration in Wistar Han Rats]

The story of the therapeutics company Acuitas is interesting because the accusation went out on twitter that the Trudeau Foundation owned 40% of Acuitas.  That was quickly debunked as false and had the fact checkers crawling all over it.  However, is it false?

Is their evidence of malfeasance? I think their is and Trudeau has “form” with other scandals. This was the main chance to get in on the ground floor and make a killing on new technology. Acuitas Therapeutics is a private company and you will never prove it as their are multiple shell companies and no transparency.  Basically they are crooks. Malone certainly thinks it is true and he is (was) an insider:

Here then is the four hour stream with the Dojo links underneath.

Dojo links with timestamps:

  • 00:00:27 Start
  • 00:17:38 Report: “[A Tissue Distribution Study of a [³H]-Labelled Lipid Nanoparticle-mRNA Formulation Containing ALC-0315 and ALC-0159 Following Intramuscular Administration in Wistar Han Rats](https://phmpt.org/wp-content/uploads/2022/03/125742_S1_M4_4223_185350.pdf)”, “SPONSOR: Acuitas Therapeutics Inc. 6190 Agronomy Road, Suite 402, Vancouver, British Columbia, V6T 1Z3 Canada”
  • 00:21:50 Neurotoxins, MPTP, Parkinson’s

 

Gigaohm Biological (27 Feb)

Gigaohm Biological (27 Feb)

Show starts at above 5 min and is worthwhile listening to Johnathan talking about his personal situation while the show is being set up.   At one point in the show Johnathan gets very emotional when someone posts in the chat that Bret Weinstein mentioned Jonathan on his popular show (Bret and Heather 116th DarkHorse Podcast Livestream: Selling out the West). So it was actually Bret who commented in the last show. He served as a professor of biology at Evergreen State College and is a podcaster and author he is among the people referred to collectively as the intellectual dark web.  Bret is one of the good guys who spoke out and it is great that Johnathan should get some recognition after losing his job and only having the support of his wife.  Let us hope that more traffic is sent Johnathan’s way. The video is divided into roughly four sections:

  1. Where are we at?
  2. The Firehose
  3. Transfection by LNP
  4. Orthornavirae

The important section for me was the mention of MSH3 Homology and Potential Recombination Link to SARS-CoV-2 Furin Cleavage Site at 53 minutes followed by the clip with Moderna CEO Steven Bancel on a News program suggesting that [Covid/FCS]  could have been a mistake (lolz)..they had a patent we warned from the very start that the virus did not have natural origins.   This was no accident.  That is like saying the twin towers were an accident.

Johnathan looks at this article A monoclonal antibody against staphylococcal enterotoxin B superantigen inhibits SARS-CoV2 entry in vitro In which they recently discovered a superantigen-like motif, similar to Staphylococcal enterotoxin B (SEB), near the S1/S2 cleavage site of SARS-CoV-2 Spike protein. And at approx 1:07 he discusses Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line which demonstrate what was said from the beginning, namely that it can enter the nucleus and change DNA. In other words it is gene therapy.

Reminder: the videos only stay up on Twitch for about six weeks but this channel tends to mirror them: https://www.bitchute.com/channel/5Wyk1zl4bD6g/

Gigaohm Biological High Resistance Low Noise Information Stream: Weekly Wrap Up and Firehose (1:41)

Gigaohm Biological (28 Jan)

Gigaohm Biological (28 Jan)

Dr Jonathan Couey is doing this show on his fiftieth birthday. This two hour show is recommended as it draws together many threads.  Why is the RNA immunization dangerous despite what we are told on TV?

1. LNPs are not proven safe to use in healthy humans FULL STOP.
2.The use of codon optimized RNA has not been show to be equivalent or robust.
3.The use of chemically altered RNA (pseudouridine) has not been adequately tested.
4. The RNA Immunizations have been shown to alter responses to other antigens.
Neither transformation nor transfection works to create sterilizing immunity.

The following papers are discussed:

Delivery of mRNA to platelets using lipid nanoparticles
"The results demonstrate that mRNA can be delivered to platelets using cLNPs(Cationic LNPs) and icLNPs (Ionizable cationic LNPs)without impairing platelet aggregation or spreading".

Hang on a minute…Platelets (thrombocytes) are colorless blood cells that help blood clot. Is this supposed to be a good thing?

Breaking the silence
"Scientists had long assumed that any genetic mutation that does not alter a protein sequence should have no impact on human health. But recent research has shown that such synonymous DNA changes can trigger disease in a number of ways. Alla Katsnelson talks to scientists and biotech companies who are speaking up about 'silent' mutations".

“It was a real mess,” Gottesman recalls. “We couldn't do it.”

Woopsie…..but meh, lets inject millions and millions and see what happens.

BNT162b Vaccine: possible condons misreading, errors in protein synthesis and alternative splicing anomalies

BNT162b2 vaccine against Covid-19 is composed of an RNA having 4284 nucleotides, divided into 6 sections, which bring the information to create a factory of S Spike proteins, the ones used by Sars-CoV-2 (Covid-19) to infect the host. After that, these proteins are directed outside the cell, triggering the immune reaction and antibody production.
The problem is the heavy alteration of the mRNA: Uracil is replaced to fool the immune system with Ψ (Pseudouridine); the letters of all codon triplets are replaced by a C or a G, to extremely increase the speed of protein synthesis; replacement of some amino acids with Proline; addition of a sequence (3'-UTR) with unknown alteration.
These impairments could cause strong doubts about the presence of codon usage errors. An eventual mistranslation has consequences on the pathophysiology of a variety of diseases.In addition, mRNA injected is a pre-mRNA, which can lead to the multiple mature mRNAs; these are alternative splicing anomalies, direct source of serious long-term harm on the human health. In essence, what will be created may not be identical with protein S Spike: just an error in translational decoding, codons misreading, production of different amino acids, then proteins, to cause serious long-term damage to human health, despite the DNA is not modified, being instead in the cell nucleus and not in the cytoplasm, where the modified mRNA arrives. However, in this case, the correlation between speed of synthesis and protein expression with synthesis errors, as well as the mechanism that could affect the translation of the sequence remain obscure, many trials have not yet been performed.

As if that was not bad enough here are some links from the comments:

Official Public Health data contains strong evidence the Fully Vaccinated are now suffering Antibody Dependent Enhancement or Acquired Immune Deficiency Syndrome; or worse both

The mRNA COVID-19 vaccine – A rare trigger of autoimmune hepatitis?

SARS-CoV-2 induces human endogenous retrovirus type W envelope protein expression in blood lymphocytes and in tissues of COVID-19 patients
 

Gigaohm Biological (28 Jan) -2 hrs

At 1:32 Dr David Wiseman giving evidence to the round table mentions pseudouridine and how it has been stabilized.This was Nearly 5 hours of testimony . Senator Ron Johnson is the only member of Congress that cares about the truth that’s being hidden by the Main Stream Media when it comes to the COVID plandemic. OAN, Rumble and Infowars will be one of the few places you can watch the COVID-19 A Second Opinion roundtable symposium in its entirety.

Someone has made a Bitchute backup channel for JC videos:

https://www.bitchute.com/channel/5Wyk1zl4bD6g/

Mistakes galore

A lot of problems are now being revealed and this is just the beginning.  A lot of people will be very upset when they learn how their trust was abused.  The next few years will see more and more injuries. Read the articles on this thread:

Immune-Mythology

Immune-Mythology

This blog article was inspired by the work that I previously did on the Wilson Vs Couey debate and by the last Gigaohm biological stream (16 Nov) which featured this video:

 

Debunking the Vaccine Makers Project video (13 min)

This is a beautifully crafted video which purports to show the science behind the mRNA vaccines, and it has gone viral among the vaccinated with lots of derogatory comments about the stupidity of the anti-vaaxers who do not understand “the science”.

This is the Disney Land fantasy version of how the “vaccines” (transfections) work. It is a fictive reality because we already know that they are not effective (hence the necessity for boosters) and have many side-effects (hence they are not safe) which points to “off-site” expression.    So, the model they are proposing is wrong.  If you had robust T cell immunity etc. (as they suggest) then you would not need boosters and if the spike was formed by the process that they show, then why is the heart etc. becoming inflamed?  My intention is to explain some of the basics and then edit and retweet the video by placing inserts to scientific articles etc.

Where does the mRNA end up?

I refer to myself as a “bucket chemist” who has done some biology (worked in plant pathology as a junior scientist) and worked for many years in the chemical industry as a Senior Chemist running a quality control lab for polymer production. The field of genetics and virology etc. is very specialized so I will break down some of these concepts for my own benefit as much as for yours.   The first point that I want to stress is that this is an experiment.   Even though they have hypotheses and are building on years of previous research there is still a lot unknown.  We are dealing with complex systems. Here follow some recent quotes in scientific papers that immediately put the video in context:

“Hence, delivery of exogeneous mRNA to the cytoplasm is essential for antigen expression, but whether this is mediated through endosomal uptake and/or direct entry through the plasma membrane is not entirely clear”. Clinical and immunological effects of mRNA vaccines in malignant diseases Heine, A., Juranek, S. & Brossart, P. Clinical and immunological effects of mRNA vaccines in malignant diseases. Mol Cancer 20, 52 (2021).

Now the following article is not about mRNA but about exosomes which can act like a pseudo-virus.  The mRNA is encapsulated in a Lipid Nano Particle and as such acts like a pseudo-virus. Who is to say that the mRNA in the cell is not also absorbed by exosomes?  Exosomes have a bi-layered lipid structure (a bit like the mRNA adjuvant) and….

“Exosomes are also able to use pathways similar to viruses to avoid lysosomal degradation. In dendritic cells internalised exosomes can bypass lysosomal degradation by being routed to a specialised, surface-accessible CD81 positive LAMP-1 negative intracellular compartment contiguous with the plasma membrane, in a manner similar to HIV-1 particles …“Another concern is the presence of naturally incorporated cellular genetic impurities with potential immunogenicity….” The exosome journey: from biogenesis to uptake and intracellular signalling Gurung, S., Perocheau, D., Touramanidou, L. et al. The exosome journey: from biogenesis to uptake and intracellular signalling. Cell Commun Signal 19, 47 (2021).

If you remember, when I quoted the paper below, it got me banned off twitter for a week:

So, according to the above paper it is entirely plausible that the exosome can absorb the S-protein…..but…but…hey….I thought it was the dendritic cell?

Off-site expression

We were told that the mRNA is injected into your arm muscle and stays there.  We know this is false form studies that showed the bio-distribution and Pharmacokinetic properties.   Japanese data shows that the spike protein of the Pfizer ‘vaccine’ gets into the blood where it circulates for several days post-vaccination and then accumulated in organs and tissues including the spleen, bone marrow, the liver, adrenal glands, and in the ovaries.

“The primary activity of a coronavirus particle is to safely deliver its genetic information (RNA) to the appropriate host cell and initiate a new infection……In addition to the viral genome and four structural proteins, it is likely that the SARS-CoV-2 particle contains host cell proteins picked up by the virus as it escapes an infected cell, but what might be included has not yet been established”. Getting to know the new coronavirus

The Moderna literature explains how it gets into the bone marrow.

Have a look at Moderna’ s promo.  It makes for an informative read but understand that mRNA is a gene therapy with potentially huge benefits for curing genetic diseases.  And that is how they sell it.   However, it also has potential for great evil.  It could be used to control populations. It could be used to engineer a sub-human servant class and a super healthy long-lived transhuman elite class (gods).  I am sure they will choose well (lolz).  Or it could (the most likely outcome in my humble opinion) go disastrously wrong and cause a mass extinction.  This technology changes the stem cells.  Despite what they say this has the chance of integrating into human DNA.  Instead of creating a super race it is more likely that we create X-men (Jewish comic book) mutants.  Generations of retards.

So, are we surprised that we get off-site expression?  That we have inflamed hearts and that it can cross the Blood Brain Barrier (BBB) potentially leading to neurodegenerative diseases?   The injection technique has a lot to do with where (and how fast) the S-protein expresses itself.  The mRNA is delivered intramuscularly but what if it is injected Intravenously? In mice it causes myopericarditis. In some people we see an almost immediate response.  Is that because it is mainlined?  Straight to the heart and boom.

Lipid nanoparticles (LNP)

A large portion of the debate between Wilson and Couey was around the adjuvant characteristic of mRNA.  Is mRNA a self-adjuvant?  Is mRNA immunogenic?  According to a Nature article “the mRNA can serve as both immunogen (encoding the viral protein) and adjuvant, owing to intrinsic immunostimulatory properties of RNA”.Wilson claimed that they solved the problem of immunogenicity by stabilising the mRNA (as pseudouridine) but as we have seen this can lead to other problems.   This is from [page 38] of the highly recommended article in IJVTPR (I have downloaded the PDF version):

So, the mRNA needs to be encased in a nanoparticle that will keep it hidden from the immune system. The second issue is getting the cells to take up the nanoparticles. This can be solved in part by incorporating phospholipids into the nanoparticle to take advantage of natural pathways of lipid particle endocytosis. The third problem is to activate the machinery that is involved in translating RNA into protein. In the case of SARS-CoV-2, the protein that is produced is the spike protein. Following spike protein synthesis, antigen-presenting cells need to present the spike protein to T cells, which will ultimately produce protective memory antibodies (Moderna, 2020). This step is not particularly straightforward, because the nanoparticles are mostly taken up by muscle cells, which, being immobile, are not necessarily equipped to launch an immune response. As we will see, the likely scenario is that the spike protein is synthesized by muscle cells and then handed over to macrophages acting as antigen-presenting cells, which then launch the standard B-cell-based antibody-generating cascade response. Worse Than the Disease? Reviewing Some Possible Unintended Consequences of the mRNA Vaccines Against COVID-19 (May 10 2021). The International Journal of Vaccine Theory, Practice, and Research (IJVTPR)Stephanie Seneff and Greg Nigh (MIT)

Now, Lipid nanoparticles (LNP) are of interest to me because we used similar products (PEG, Span, Tween) in polymerizations for creating micelles. This is because we made emulsions using oil and water phases and oil and water do not mix so you need to use surfactants (soaps) to create micelles:

The surfactant is depicted looking like a tadpole with a long tail and a functional “head”.  It all depends what charge (polarity) the head and the tail have as to whether it attracts or repels the water (hydrophobic or hydrophilic).  The polymerization happened inside the micelle.  This is what I found immensely interesting (recommended if interested in chemistry):

“As mentioned above, cationic lipids are used to formulate LNPs containing nucleic acids [17]. Cationic amino groups within these lipids interact with nucleic acids' negatively charged phosphate groups, resulting in engraftment in an LNP. In 1989, a lipoplex
structure containing synthetic cationic lipid DOTMA (N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride) and helper lipid DOPE (dioleoylphosphatidylethanolamine)was used to generate Luc mRNA LNPs that successfully transfected several cell types [41].
Further, in vitro transfections have long used cationic lipids including commercially available Lipofectamine, which is widely used for RNA and DNA in vitro transfections despite its known cytotoxicity [42]. While separated, both cationic and anionic lipids in cell membranes display a cylindrical shape, which supports bilayer structure formation. However,when these lipids interact together via negatively and positively charged headgroups, they
form cone-shaped structures that promote hexagonal 1--111 phase formation. This hexagonal phase disorganizes bilayer structures and correlates with membrane fusion as well as the disruption that is partially responsible for cationic lipid toxicity [43]. When systemically delivered, LNPs with permanent surface charge interact with serum proteins, and this inter- action causes rapid clearance from the circulation [44,45]. Indeed, cationic LNPs have been shown to generate toxicity towards phagocytic cells in vitro. [46]. Additionally, systemically delivering cationic LNPs induces a strong immune response by activating the interferon type I response and instigating expression of INFY, and the pro-inflammatory cytokine IL-2. [47]. Excessive immune reaction to LNPs is not desirable because uncontrolled cytokine release can lead to life-threating conditions……” Lipid Nanoparticles for Organ-Specific mRNA Therapeutic Deliverẏ Zak, M.M.; Zangi, L. Lipid Pharmaceutics 2021, 13, 1675.https://doi.org/10.3390/pharmaceutics13101675

The bold emphasis is mine.  You get the picture…. a lot can go wrong.  When it happens in a reactor and the reactor goes solid you just dig it out and clean it.  Not so easy in a human brain (lolz).  All joking aside these molecules have different characteristics at different molecular weights and disassociate differently depending on pH.  Apparently, they can be made to target different organs depending on those characteristics.  It all works well in theory.  In practice when we did stuff on the plant, and it went horribly wrong and blew a reactor up the research department said…. well, that never happened in the lab (or worse still) …it only happened once, we thought it wouldn’t be a problem (lolz).  We cleaned the glasswork, and it was all Hunky Dory. A lot can go wrong (and that is leaving aside the anaphylactic shock that is often triggered by these compounds).

So much of the biochemistry is modelled by computers based on mathematics and chemical properties of proteins etc.  One such open-source model is Martini 3 (which sounds like a bunch of drunk scientists to me).  While it is true that electron microscopy and tomography etc. is used, there is still much that is speculative.

“The viral envelope is flexible and lacks symmetry. The structures of E, M and S proteins have not been resolved experimentally. There are some structural insights available on S trimers and E pentamers, but there is no accurate model of the M dimer…..Moreover, the nature of the interactions between these three proteins is believed to be more complex than originally expected. The complexity and plasticity of the virus make it challenging to study the structure of the viral envelope”.                                                    https://www.news-medical.net/news/20210920/Scientists-simulate-SARS-CoV-2-virion-envelope-using-Martini-3.aspx

The furin cleavage site

The virus was man made and originated in a lab. It was deliberately released to provide cover for collapsing the system and bringing in the NWO. I know who did it and why. Suffice to say it was not just China. There were global institutes involved and this is driven by the bankers. This article is not going to go into that, but I did want to provide a few pointers for those (like me) who are completely lost in all the technical jargon etc. So, some simple basics.

All the fuss is around the furin cleavage site which so far is different from any animal version. It is a combination of different animal cleavage sites (bat and pangolin). Furin should be understood as the scissors that cuts the cleavage site near the Receptor Binding Domain (RBD). SARS-CoV-2 entry requires sequential cleavage of the spike glycoprotein at the S1/S2 and the S2ʹ cleavage sites to mediate membrane fusion. SARS-CoV-2 has a polybasic insertion (PRRAR) at the S1/S2 cleavage site that can be cleaved by furin.

Furin is a protease enzyme that in humans and other animals is encoded by the FURIN gene. Some proteins are inactive when they are first synthesized, and must have sections removed in order to become active. Furin cleaves these sections and activates the proteins. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg'). In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza, dengue fever, several filoviruses including ebola and marburg virus, and the spike protein of SARS-CoV-2, must be cleaved by furin or furin-like proteases to become fully functional.

PRRA and ccu cgg cgg gca

The furin cleavage site consists of four amino acids PRRA, which are encoded by 12 inserted nucleotides in the S gene. A characteristic feature of this site is an arginine doublet. This insertion could have occurred by random insertion mutation, recombination or by laboratory insertion. The researchers say the possibility of random insertion is too low to explain the origin of this motif.
Surprisingly, the CGGCGG codons encoding the two arginines of the doublet in SARS-CoV-2 are not found in any of the furin sites in other viral proteins expressed by a wide range of viruses.
Even within the SARS-CoV-2, where arginine is encoded by six codons, only a minority of arginine residues are encoded by the CGG codon. Again, only two of the 42 arginines in the SARS-CoV-2 spike are encoded by this codon – and these are in the PRRA motif.
For recombination to occur, there must be a donor, from another furin site and probably from another virus. In the absence of a known virus containing this arginine doublet encoded by the CGGCGG codons, the researchers discount the recombination theory as the mechanism underlying the emergence of PRRA in SARS-CoV-2.
https://www.news-medical.net/news/20210217/The-origin-of-SARS-CoV-2-furin-cleavage-site-remains-a-mystery.aspx
This genetic material, called RNA, and which some viruses inherit from others, works like an instruction manual for manufacturing the proteins that form SARS-CoV-2. The genome of the new coronavirus has around 30,000 letters with enough instructions to penetrate a cell, hijack its machinery and make thousands of copies of itself. The instructions for the human cell to manufacture the virus’s main weapon – its spike protein used as a key by new viruses to gain access to more and more cells – is contained in around 4,000 letters. The coronavirus spike protein is like a double-faceted key. It first latches onto the lock – the human cell’s ACE2 receptor. Its next step is to control the binding of the virus’s membrane to the cell’s membrane. The main difference between SARS-CoV-2 and other coronaviruses is the appearance of 12 extra letters in its genome. The experts flag up this extremely short sequence as the main culprit regarding its virulence.

With some exceptions, a three-nucleotide codon in a nucleic acid sequence specifies a single amino acid. In other words, the mRNA inside the corona virus codes for the amino acids in the DNA.  There are four nitrogenous bases that occur in DNA molecules: cytosine, guanine, adenine, and thymine (abbreviated as C, G, A, and T). RNA molecules contain cytosine, guanine, and adenine, but they have a different nitrogenous base, uracil (U) instead of thymine. The building blocks of DNA are nucleotides, which are made up of three parts: a deoxyribose (5-carbon sugar), a phosphate group, and a nitrogenous base. It provides the instructions. These are the twelve letters (four sets of three condons) that changed the world ccu cgg cgg gca ……….. c=cytosine, u=uracil, g=guanine, a=adenine and this is what they code for:

  • ccu > ENCODES > P (Proline)
  • cgg > ENCODES > R (Arginine)
  • cgg > ENCODES > R (Arginine)
  • gca > ENCODES > A (Alanine)

 

Gigaohm Biological (16 Nov)

Gigaohm Biological (16 Nov)

Recommended

Another Excellent show by Jonathan.  I am going to download this video and make clips.  Very good analysis of the biology and good social commentary.  I can hardly keep up with all the material that is in-coming but I am still busy transcribing the Couey vs. Wilson debate and I have also begun dissecting the viral video clip (that Jonathan includes in this presentation).

Watch out for the next blog article it should go up sometime today.  It will be a commentary on the clip that Jonathan analyzes…..  coming soon..

Gigaohm Biological (16 Nov) (2:38)