Tag: off-site expression

Immune-Mythology

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Immune-Mythology

This blog article was inspired by the work that I previously did on the Wilson Vs Couey debate and by the last Gigaohm biological stream (16 Nov) which featured this video:

 

Debunking the Vaccine Makers Project video (13 min)

This is a beautifully crafted video which purports to show the science behind the mRNA vaccines, and it has gone viral among the vaccinated with lots of derogatory comments about the stupidity of the anti-vaaxers who do not understand “the science”.

This is the Disney Land fantasy version of how the “vaccines” (transfections) work. It is a fictive reality because we already know that they are not effective (hence the necessity for boosters) and have many side-effects (hence they are not safe) which points to “off-site” expression.    So, the model they are proposing is wrong.  If you had robust T cell immunity etc. (as they suggest) then you would not need boosters and if the spike was formed by the process that they show, then why is the heart etc. becoming inflamed?  My intention is to explain some of the basics and then edit and retweet the video by placing inserts to scientific articles etc.

Where does the mRNA end up?

I refer to myself as a “bucket chemist” who has done some biology (worked in plant pathology as a junior scientist) and worked for many years in the chemical industry as a Senior Chemist running a quality control lab for polymer production. The field of genetics and virology etc. is very specialized so I will break down some of these concepts for my own benefit as much as for yours.   The first point that I want to stress is that this is an experiment.   Even though they have hypotheses and are building on years of previous research there is still a lot unknown.  We are dealing with complex systems. Here follow some recent quotes in scientific papers that immediately put the video in context:

“Hence, delivery of exogeneous mRNA to the cytoplasm is essential for antigen expression, but whether this is mediated through endosomal uptake and/or direct entry through the plasma membrane is not entirely clear”. Clinical and immunological effects of mRNA vaccines in malignant diseases Heine, A., Juranek, S. & Brossart, P. Clinical and immunological effects of mRNA vaccines in malignant diseases. Mol Cancer 20, 52 (2021).

Now the following article is not about mRNA but about exosomes which can act like a pseudo-virus.  The mRNA is encapsulated in a Lipid Nano Particle and as such acts like a pseudo-virus. Who is to say that the mRNA in the cell is not also absorbed by exosomes?  Exosomes have a bi-layered lipid structure (a bit like the mRNA adjuvant) and….

“Exosomes are also able to use pathways similar to viruses to avoid lysosomal degradation. In dendritic cells internalised exosomes can bypass lysosomal degradation by being routed to a specialised, surface-accessible CD81 positive LAMP-1 negative intracellular compartment contiguous with the plasma membrane, in a manner similar to HIV-1 particles …“Another concern is the presence of naturally incorporated cellular genetic impurities with potential immunogenicity….” The exosome journey: from biogenesis to uptake and intracellular signalling Gurung, S., Perocheau, D., Touramanidou, L. et al. The exosome journey: from biogenesis to uptake and intracellular signalling. Cell Commun Signal 19, 47 (2021).

If you remember, when I quoted the paper below, it got me banned off twitter for a week:

So, according to the above paper it is entirely plausible that the exosome can absorb the S-protein…..but…but…hey….I thought it was the dendritic cell?

Off-site expression

We were told that the mRNA is injected into your arm muscle and stays there.  We know this is false form studies that showed the bio-distribution and Pharmacokinetic properties.   Japanese data shows that the spike protein of the Pfizer ‘vaccine’ gets into the blood where it circulates for several days post-vaccination and then accumulated in organs and tissues including the spleen, bone marrow, the liver, adrenal glands, and in the ovaries.

“The primary activity of a coronavirus particle is to safely deliver its genetic information (RNA) to the appropriate host cell and initiate a new infection……In addition to the viral genome and four structural proteins, it is likely that the SARS-CoV-2 particle contains host cell proteins picked up by the virus as it escapes an infected cell, but what might be included has not yet been established”. Getting to know the new coronavirus

The Moderna literature explains how it gets into the bone marrow.

Have a look at Moderna’ s promo.  It makes for an informative read but understand that mRNA is a gene therapy with potentially huge benefits for curing genetic diseases.  And that is how they sell it.   However, it also has potential for great evil.  It could be used to control populations. It could be used to engineer a sub-human servant class and a super healthy long-lived transhuman elite class (gods).  I am sure they will choose well (lolz).  Or it could (the most likely outcome in my humble opinion) go disastrously wrong and cause a mass extinction.  This technology changes the stem cells.  Despite what they say this has the chance of integrating into human DNA.  Instead of creating a super race it is more likely that we create X-men (Jewish comic book) mutants.  Generations of retards.

So, are we surprised that we get off-site expression?  That we have inflamed hearts and that it can cross the Blood Brain Barrier (BBB) potentially leading to neurodegenerative diseases?   The injection technique has a lot to do with where (and how fast) the S-protein expresses itself.  The mRNA is delivered intramuscularly but what if it is injected Intravenously? In mice it causes myopericarditis. In some people we see an almost immediate response.  Is that because it is mainlined?  Straight to the heart and boom.

Lipid nanoparticles (LNP)

A large portion of the debate between Wilson and Couey was around the adjuvant characteristic of mRNA.  Is mRNA a self-adjuvant?  Is mRNA immunogenic?  According to a Nature article “the mRNA can serve as both immunogen (encoding the viral protein) and adjuvant, owing to intrinsic immunostimulatory properties of RNA”.Wilson claimed that they solved the problem of immunogenicity by stabilising the mRNA (as pseudouridine) but as we have seen this can lead to other problems.   This is from [page 38] of the highly recommended article in IJVTPR (I have downloaded the PDF version):

So, the mRNA needs to be encased in a nanoparticle that will keep it hidden from the immune system. The second issue is getting the cells to take up the nanoparticles. This can be solved in part by incorporating phospholipids into the nanoparticle to take advantage of natural pathways of lipid particle endocytosis. The third problem is to activate the machinery that is involved in translating RNA into protein. In the case of SARS-CoV-2, the protein that is produced is the spike protein. Following spike protein synthesis, antigen-presenting cells need to present the spike protein to T cells, which will ultimately produce protective memory antibodies (Moderna, 2020). This step is not particularly straightforward, because the nanoparticles are mostly taken up by muscle cells, which, being immobile, are not necessarily equipped to launch an immune response. As we will see, the likely scenario is that the spike protein is synthesized by muscle cells and then handed over to macrophages acting as antigen-presenting cells, which then launch the standard B-cell-based antibody-generating cascade response. Worse Than the Disease? Reviewing Some Possible Unintended Consequences of the mRNA Vaccines Against COVID-19 (May 10 2021). The International Journal of Vaccine Theory, Practice, and Research (IJVTPR)Stephanie Seneff and Greg Nigh (MIT)

Now, Lipid nanoparticles (LNP) are of interest to me because we used similar products (PEG, Span, Tween) in polymerizations for creating micelles. This is because we made emulsions using oil and water phases and oil and water do not mix so you need to use surfactants (soaps) to create micelles:

The surfactant is depicted looking like a tadpole with a long tail and a functional “head”.  It all depends what charge (polarity) the head and the tail have as to whether it attracts or repels the water (hydrophobic or hydrophilic).  The polymerization happened inside the micelle.  This is what I found immensely interesting (recommended if interested in chemistry):

“As mentioned above, cationic lipids are used to formulate LNPs containing nucleic acids [17]. Cationic amino groups within these lipids interact with nucleic acids' negatively charged phosphate groups, resulting in engraftment in an LNP. In 1989, a lipoplex
structure containing synthetic cationic lipid DOTMA (N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride) and helper lipid DOPE (dioleoylphosphatidylethanolamine)was used to generate Luc mRNA LNPs that successfully transfected several cell types [41].
Further, in vitro transfections have long used cationic lipids including commercially available Lipofectamine, which is widely used for RNA and DNA in vitro transfections despite its known cytotoxicity [42]. While separated, both cationic and anionic lipids in cell membranes display a cylindrical shape, which supports bilayer structure formation. However,when these lipids interact together via negatively and positively charged headgroups, they
form cone-shaped structures that promote hexagonal 1--111 phase formation. This hexagonal phase disorganizes bilayer structures and correlates with membrane fusion as well as the disruption that is partially responsible for cationic lipid toxicity [43]. When systemically delivered, LNPs with permanent surface charge interact with serum proteins, and this inter- action causes rapid clearance from the circulation [44,45]. Indeed, cationic LNPs have been shown to generate toxicity towards phagocytic cells in vitro. [46]. Additionally, systemically delivering cationic LNPs induces a strong immune response by activating the interferon type I response and instigating expression of INFY, and the pro-inflammatory cytokine IL-2. [47]. Excessive immune reaction to LNPs is not desirable because uncontrolled cytokine release can lead to life-threating conditions……” Lipid Nanoparticles for Organ-Specific mRNA Therapeutic Deliverẏ Zak, M.M.; Zangi, L. Lipid Pharmaceutics 2021, 13, 1675.https://doi.org/10.3390/pharmaceutics13101675

The bold emphasis is mine.  You get the picture…. a lot can go wrong.  When it happens in a reactor and the reactor goes solid you just dig it out and clean it.  Not so easy in a human brain (lolz).  All joking aside these molecules have different characteristics at different molecular weights and disassociate differently depending on pH.  Apparently, they can be made to target different organs depending on those characteristics.  It all works well in theory.  In practice when we did stuff on the plant, and it went horribly wrong and blew a reactor up the research department said…. well, that never happened in the lab (or worse still) …it only happened once, we thought it wouldn’t be a problem (lolz).  We cleaned the glasswork, and it was all Hunky Dory. A lot can go wrong (and that is leaving aside the anaphylactic shock that is often triggered by these compounds).

So much of the biochemistry is modelled by computers based on mathematics and chemical properties of proteins etc.  One such open-source model is Martini 3 (which sounds like a bunch of drunk scientists to me).  While it is true that electron microscopy and tomography etc. is used, there is still much that is speculative.

“The viral envelope is flexible and lacks symmetry. The structures of E, M and S proteins have not been resolved experimentally. There are some structural insights available on S trimers and E pentamers, but there is no accurate model of the M dimer…..Moreover, the nature of the interactions between these three proteins is believed to be more complex than originally expected. The complexity and plasticity of the virus make it challenging to study the structure of the viral envelope”.                                                    https://www.news-medical.net/news/20210920/Scientists-simulate-SARS-CoV-2-virion-envelope-using-Martini-3.aspx

The furin cleavage site

The virus was man made and originated in a lab. It was deliberately released to provide cover for collapsing the system and bringing in the NWO. I know who did it and why. Suffice to say it was not just China. There were global institutes involved and this is driven by the bankers. This article is not going to go into that, but I did want to provide a few pointers for those (like me) who are completely lost in all the technical jargon etc. So, some simple basics.

All the fuss is around the furin cleavage site which so far is different from any animal version. It is a combination of different animal cleavage sites (bat and pangolin). Furin should be understood as the scissors that cuts the cleavage site near the Receptor Binding Domain (RBD). SARS-CoV-2 entry requires sequential cleavage of the spike glycoprotein at the S1/S2 and the S2ʹ cleavage sites to mediate membrane fusion. SARS-CoV-2 has a polybasic insertion (PRRAR) at the S1/S2 cleavage site that can be cleaved by furin.

Furin is a protease enzyme that in humans and other animals is encoded by the FURIN gene. Some proteins are inactive when they are first synthesized, and must have sections removed in order to become active. Furin cleaves these sections and activates the proteins. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Furin cleaves proteins just downstream of a basic amino acid target sequence (canonically, Arg-X-(Arg/Lys) -Arg'). In addition to processing cellular precursor proteins, furin is also utilized by a number of pathogens. For example, the envelope proteins of viruses such as HIV, influenza, dengue fever, several filoviruses including ebola and marburg virus, and the spike protein of SARS-CoV-2, must be cleaved by furin or furin-like proteases to become fully functional.

PRRA and ccu cgg cgg gca

The furin cleavage site consists of four amino acids PRRA, which are encoded by 12 inserted nucleotides in the S gene. A characteristic feature of this site is an arginine doublet. This insertion could have occurred by random insertion mutation, recombination or by laboratory insertion. The researchers say the possibility of random insertion is too low to explain the origin of this motif.
Surprisingly, the CGGCGG codons encoding the two arginines of the doublet in SARS-CoV-2 are not found in any of the furin sites in other viral proteins expressed by a wide range of viruses.
Even within the SARS-CoV-2, where arginine is encoded by six codons, only a minority of arginine residues are encoded by the CGG codon. Again, only two of the 42 arginines in the SARS-CoV-2 spike are encoded by this codon – and these are in the PRRA motif.
For recombination to occur, there must be a donor, from another furin site and probably from another virus. In the absence of a known virus containing this arginine doublet encoded by the CGGCGG codons, the researchers discount the recombination theory as the mechanism underlying the emergence of PRRA in SARS-CoV-2.
https://www.news-medical.net/news/20210217/The-origin-of-SARS-CoV-2-furin-cleavage-site-remains-a-mystery.aspx
This genetic material, called RNA, and which some viruses inherit from others, works like an instruction manual for manufacturing the proteins that form SARS-CoV-2. The genome of the new coronavirus has around 30,000 letters with enough instructions to penetrate a cell, hijack its machinery and make thousands of copies of itself. The instructions for the human cell to manufacture the virus’s main weapon – its spike protein used as a key by new viruses to gain access to more and more cells – is contained in around 4,000 letters. The coronavirus spike protein is like a double-faceted key. It first latches onto the lock – the human cell’s ACE2 receptor. Its next step is to control the binding of the virus’s membrane to the cell’s membrane. The main difference between SARS-CoV-2 and other coronaviruses is the appearance of 12 extra letters in its genome. The experts flag up this extremely short sequence as the main culprit regarding its virulence.

With some exceptions, a three-nucleotide codon in a nucleic acid sequence specifies a single amino acid. In other words, the mRNA inside the corona virus codes for the amino acids in the DNA.  There are four nitrogenous bases that occur in DNA molecules: cytosine, guanine, adenine, and thymine (abbreviated as C, G, A, and T). RNA molecules contain cytosine, guanine, and adenine, but they have a different nitrogenous base, uracil (U) instead of thymine. The building blocks of DNA are nucleotides, which are made up of three parts: a deoxyribose (5-carbon sugar), a phosphate group, and a nitrogenous base. It provides the instructions. These are the twelve letters (four sets of three condons) that changed the world ccu cgg cgg gca ……….. c=cytosine, u=uracil, g=guanine, a=adenine and this is what they code for:

  • ccu > ENCODES > P (Proline)
  • cgg > ENCODES > R (Arginine)
  • cgg > ENCODES > R (Arginine)
  • gca > ENCODES > A (Alanine)