Stop Condons

Stop Condons

It is becoming clear that enormous risks have been taken with this experimental procedure which is the precursor platform to gene therapy.  The Fourth Industrial Revolution is riding on this new technology and they have pushed ahead despite all the unknowns. In order to make it work they had to stabilize the mRNA otherwise it would be degraded by the body. They did this by methylating the uridine to pseudouridine.

However, this can cause other problems such as reading through “stop condons”.  If you have no idea what I am talking about I suggest you look at this previous article and specifically watch the video at the end with Dr Kevin McKernan and Dr. Jay Couey.

Sherlock Ohmes

 

A cell replicates itself using its genomic intels. To do this, it reproduces what is available in DNA, until the stop codon (red) is met. The orange area is the most important part to get a perfect clone that does exactly what it is intended to do but, there is a way for the 3′-UTR (purple) to be included in the duplicated cell, which produces a cell that does something else. Something unknown.

3′-UTR is the Three prime untranslated region . In molecular genetics, the three prime untranslated region (3′-UTR) is the section of messenger RNA (mRNA) that immediately follows the translation termination codon. The 3′-UTR often contains regulatory regions that post-transcriptionally influence gene expression.

Putting on the brakes

In order to prevent this happening they have put more than one stop condon in.

Why use two stop UGA codons instead of one in the spike protein mRNA for the BioNTech/Pfizer SARS-CoV-2 vaccine?

 

Read Through

People have valid concerns that “read through ” will occur on the stop condons and it is known that in certain circumstances antibiotics can cause this. Here are some comments discussing the problem.

RefSeq curation and annotation of stop codon recoding in vertebrates

So it’s the alteration from uridine to pseudouridine allowing the codons to be read in the ribosome to instruct it to make the artificial S protein, but in the very act of that alteration, it screws up the “genetic compiler”, thereby misreading the code and translating the 3’UTR? There is a good chance that at least some (how many?) will readthrough causing the 3_UTR to be translated and attached to Spike Protein. And then that could cause Spike to mis-fold somehow (on this see McKernan). But NO testing was ever done to see what happens. They didn’t want to know.

If they already had their perfect 5’UTR in 2019 they wouldn’t have had to test 280,000 new ones, but what do I know eh?

OAS and ADE

Original Antigenic Sin and Antibody Dependent Enhancement.

 

The “original antigenic sin” and its relevance for SARS-CoV-2 (COVID-19) vaccination

Conclusion

What would I know?   I am not a geneticist just a bucket chemist. However, I know enough to hear alarm bells sounding. Especially when pharma hides data.  Especially when they have indemnity.

Biohazard Alarm[WARNING: The sound is very LOUD] 49secs

Gigaohm Biological (28 Jan)

Gigaohm Biological (28 Jan)

Dr Jonathan Couey is doing this show on his fiftieth birthday. This two hour show is recommended as it draws together many threads.  Why is the RNA immunization dangerous despite what we are told on TV?

1. LNPs are not proven safe to use in healthy humans FULL STOP.
2.The use of codon optimized RNA has not been show to be equivalent or robust.
3.The use of chemically altered RNA (pseudouridine) has not been adequately tested.
4. The RNA Immunizations have been shown to alter responses to other antigens.
Neither transformation nor transfection works to create sterilizing immunity.

The following papers are discussed:

Delivery of mRNA to platelets using lipid nanoparticles
"The results demonstrate that mRNA can be delivered to platelets using cLNPs(Cationic LNPs) and icLNPs (Ionizable cationic LNPs)without impairing platelet aggregation or spreading".

Hang on a minute…Platelets (thrombocytes) are colorless blood cells that help blood clot. Is this supposed to be a good thing?

Breaking the silence
"Scientists had long assumed that any genetic mutation that does not alter a protein sequence should have no impact on human health. But recent research has shown that such synonymous DNA changes can trigger disease in a number of ways. Alla Katsnelson talks to scientists and biotech companies who are speaking up about 'silent' mutations".

“It was a real mess,” Gottesman recalls. “We couldn't do it.”

Woopsie…..but meh, lets inject millions and millions and see what happens.

BNT162b Vaccine: possible condons misreading, errors in protein synthesis and alternative splicing anomalies

BNT162b2 vaccine against Covid-19 is composed of an RNA having 4284 nucleotides, divided into 6 sections, which bring the information to create a factory of S Spike proteins, the ones used by Sars-CoV-2 (Covid-19) to infect the host. After that, these proteins are directed outside the cell, triggering the immune reaction and antibody production.
The problem is the heavy alteration of the mRNA: Uracil is replaced to fool the immune system with Ψ (Pseudouridine); the letters of all codon triplets are replaced by a C or a G, to extremely increase the speed of protein synthesis; replacement of some amino acids with Proline; addition of a sequence (3'-UTR) with unknown alteration.
These impairments could cause strong doubts about the presence of codon usage errors. An eventual mistranslation has consequences on the pathophysiology of a variety of diseases.In addition, mRNA injected is a pre-mRNA, which can lead to the multiple mature mRNAs; these are alternative splicing anomalies, direct source of serious long-term harm on the human health. In essence, what will be created may not be identical with protein S Spike: just an error in translational decoding, codons misreading, production of different amino acids, then proteins, to cause serious long-term damage to human health, despite the DNA is not modified, being instead in the cell nucleus and not in the cytoplasm, where the modified mRNA arrives. However, in this case, the correlation between speed of synthesis and protein expression with synthesis errors, as well as the mechanism that could affect the translation of the sequence remain obscure, many trials have not yet been performed.

As if that was not bad enough here are some links from the comments:

Official Public Health data contains strong evidence the Fully Vaccinated are now suffering Antibody Dependent Enhancement or Acquired Immune Deficiency Syndrome; or worse both

The mRNA COVID-19 vaccine – A rare trigger of autoimmune hepatitis?

SARS-CoV-2 induces human endogenous retrovirus type W envelope protein expression in blood lymphocytes and in tissues of COVID-19 patients
 

Gigaohm Biological (28 Jan) -2 hrs

At 1:32 Dr David Wiseman giving evidence to the round table mentions pseudouridine and how it has been stabilized.This was Nearly 5 hours of testimony . Senator Ron Johnson is the only member of Congress that cares about the truth that’s being hidden by the Main Stream Media when it comes to the COVID plandemic. OAN, Rumble and Infowars will be one of the few places you can watch the COVID-19 A Second Opinion roundtable symposium in its entirety.

Someone has made a Bitchute backup channel for JC videos:

https://www.bitchute.com/channel/5Wyk1zl4bD6g/

Mistakes galore

A lot of problems are now being revealed and this is just the beginning.  A lot of people will be very upset when they learn how their trust was abused.  The next few years will see more and more injuries. Read the articles on this thread:

Sherlock Ohmes

Sherlock Ohmes

Sherlock Ohmes (Jonathan  from Gigaohm Biological) discusses the curious case of the virus that did not infect.  This is a 20 minute clip from the previous show (24 Jan)  which I have saved on my Bitchute channel because it is an important part of  the Omicron sleuthing being done hence the corny Sherlock Ohmes reference and the curious case of the dog that did not bark.  In that case the dog did not bark because it knew the thief.  In this case the virus did not infect because they did not test for it.  If you don’t test for it, then you don’t find it.

Gigaohm Biological and Ethical Skeptic (20 min)

Here is previous work by the Ethical Skeptic in case you missed it.

Omicron sleuths

 


Here is Julius Ruechel on the disappearance of influenza

Here are the scientific articles:

BNT162b2 Vaccine: Possible Codons Misreading, Errors in Protein Synthesis and Alternative Splicing’s Anomalies

Translation affects mRNA stability in a codon-dependent manner in human cells

Gamma-Glutamyltransferase: A Predictive Biomarker of Cellular Antioxidant Inadequacy and Disease Risk

Innate Immune Suppression by SARS-CoV-2 mRNA Vaccinations: The role of G-quadruplexes, exosomes and microRNAs

Kevin McKernan Dr. Jay Couey (1:07)

I finally found the episode with Kevin McKernan  (not Kevin McCairn) and RNA Immunization Tech: Some Things You Should Know.  This was aired on the 20th of December and was on Vimeo.  I have downloaded it and placed it on Bitchute.  Kevin McKernan discusses  with Dr. Jay Couey sequences and consequences of mRna transfections.

Think of stop condons as traffic lights.  Sometimes you can run them and you will be OK but if you are unlucky there will be a huge truck going through the intersection. Here is the blog post discussing pseudouridine  using Kevin McKernan’s tweets:

mRNA stability

Here is an explanation about the Furin Cleavage Site (FCS) and condons, etc:

Immune-Mythology

 

 

mRNA stability

mRNA stability

This post was inspired by the Couey vs Wilson debate which I am still transcribing and which can be found here:

Couey vs Wilson debate

The debate got rather heated and complex on the question of whether or not mRNA is an adjuvant or a self-adjuvant as their are studies that indicate that it could be used as an adjuvant.  Now, an adjuvant is a compound or chemical that irritates or triggers the immune system.

All vaccines have adjuvants and Dr Wilson (citing a scientific study) showed that mRNA could act as a self-adjuvant.    In other words there would be no need for the addition of an adjuvant.    The objection voiced by Jonathan was that mRNA is highly immunogenic. In other words it could trigger all sorts of autoimmune diseases and inflamation, especially if it was free floating in the blood.

In order to prevent this they need to do two things 1. encapsulate the mRNA in a Lipid Nano Particle (LNP)  and 2. stabilize the mRNA.  It turns out they do use PEG and polysorbate 80 as adjuvants.

 

PEG is a long chain polymer and its characteristics are different depending on the length of the polymer chain.  Polysorbate 80 is similar to PEG.   I have useed these substances as surfactants etc when making polymers. They have certain hydrophobic and hydrophilic qualities which can be used when mixing aqueous and oil phases and in micelle formation. The above paper lists MF59 adjuvant as an emulsion adjuvant composed of an oil phase (squalene 4.3%): and an aqueous phase (polysorbate 80:0.5%, sorbitan trioleate 0.5%). These vaccines do use an adjuvant, therefore Dr Wilson was incorrect.

Polyethylene glycol

A PEGylated lipid is used as an excipient in both the Moderna and Pfizer–BioNTech vaccines for SARS-CoV-2. Both RNA vaccines consist of messenger RNA, or mRNA, encased in a bubble of oily molecules called lipids. Proprietary lipid technology is used for each. In both vaccines, the bubbles are coated with a stabilizing molecule of polyethylene glycol.[medical citation needed] As of December 2020 there is some concern that PEG could trigger allergic reaction, and in fact allergic reactions are the driver for both the United Kingdom and Canadian regulators to issue an advisory, noting that: two “individuals in the U.K… were treated and have recovered” from anaphylactic shock. As of 18 December, the US CDC stated that in their jurisdiction six cases of “severe allergic reaction” had been recorded from more than 250,000 vaccinations, and of those six only one person had a “history of vaccination reactions”

Two things to mention here, firstly, many of the anaphylactic reactions are cause by the adjuvants like PEG and secondly, PEG and other polymers are being examined as delivery mechanisms for drugs because they cross the Blood Brain Barrier (BBB). If that is the case then they should also be able to deliver the spike protein across the BBB and evidence is emerging that happens.  That is not a good thing. There is a reason the brain is protected and segregated from the circulatory system and while it might be desirable to cross the BBB to treat diseases like dementia and Parkinson’s it comes with a risk.

So, as far as the debate is concerned Jonathan is correct. The vaccines do have an adjuvant and it is a LNP.  Note that the Wikipedia quote above uses the word excipient rather than adjuvant. An excipient is an inactive substance ….but is it really inactive? How can it be “inactive” when PEG antibodies are found and when some people suffer anaphylactic shock?

Polyethylene Glycol Immunogenicity: Theoretical, Clinical, and Practical Aspects of Anti-Polyethylene Glycol Antibodies

Pseudouridine

Wilson claimed that the self-adjuvant effect of mRNA was due to the pseudourodilatation of the mRNA. Pseudouridine is supposed to be more stable.

Ψ (pseudouridine) is also found in mRNAs which are the template for protein synthesis. Ψ residues in mRNA can affect the coding specificity of stop codons UAA, UGA, and UAG. In these stop codons both a U→Ψ modification and a U→C mutation both promote nonsense suppression.[6] In the SARS-CoV2 vaccine from BioNTech/Pfizer, also known as BNT162b2, Tozinameran or Comirnaty, all U's have been substituted with N1-methylpseudouridine,[7] a nucleoside related to Ψ that contains a methyl group added to N1 atom.

This is the tweet (second one Tishbite) that I sent out (well worth reading):

 

They’ve fiddled around with codon optimization to increase stability, but that translates into (1) enormous quantities produced of spike and (2) a different spike protein which could be even more pathogenic.

 

In case the above tweet disappears the link takes you here:

https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/

They are making people disappear off twitter (and FB)….this guy is good:

Conclusion

None of that fills me with confidence especially the fact that it can misfold.  That can lead to prions and neurodegeneration. Expect to see more diseases of the Central Nervous System,  dementia, Parkinson and CJD as well as more strokes, cancers and heart attacks. Don’t let them go anywhere near the kids with this.  We have been warning for more than a year and a half.  Is anyone listening?