{"id":24704,"date":"2022-02-04T06:06:39","date_gmt":"2022-02-04T06:06:39","guid":{"rendered":"https:\/\/www.biblaridion.info\/blog\/?p=24704"},"modified":"2022-02-04T06:06:39","modified_gmt":"2022-02-04T06:06:39","slug":"stop-condons","status":"publish","type":"post","link":"https:\/\/www.biblaridion.info\/blog\/stop-condons\/","title":{"rendered":"Stop Condons"},"content":{"rendered":"<h1>Stop Condons<\/h1>\n<p>It is becoming clear that enormous risks have been taken with this experimental procedure which is the precursor platform to gene therapy.\u00a0 The <em>Fourth Industrial Revolution<\/em> is riding on this new technology and they have pushed ahead despite all the unknowns. In order to make it work they had to stabilize the mRNA otherwise it would be degraded by the body. They did this by methylating the uridine to pseudouridine.<\/p>\n<blockquote class=\"twitter-tweet\" data-theme=\"dark\">\n<p dir=\"ltr\" lang=\"en\">methylation of the uridine to pseudouridine <a href=\"https:\/\/t.co\/fIQMvfsvHg\">pic.twitter.com\/fIQMvfsvHg<\/a><\/p>\n<p>\u2014 The Tishbite (@Tishbite_redux) <a href=\"https:\/\/twitter.com\/Tishbite_redux\/status\/1489468916799660036?ref_src=twsrc%5Etfw\">February 4, 2022<\/a><\/p><\/blockquote>\n<p><script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script><\/p>\n<p>However, this can cause other problems such as reading through &#8220;stop condons&#8221;.\u00a0 If you have no idea what I am talking about I suggest you look at this previous article and specifically watch the video at the end with Dr Kevin McKernan and Dr. Jay Couey.<\/p>\n<blockquote class=\"wp-embedded-content\" data-secret=\"9a9FNV3uVj\"><p><a href=\"https:\/\/www.biblaridion.info\/blog\/immune-mythology\/\">Immune-Mythology<\/a><\/p><\/blockquote>\n<p><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; clip: rect(1px, 1px, 1px, 1px);\" title=\"&#8220;Immune-Mythology&#8221; &#8212; The Apocalypse and Daniel\" src=\"https:\/\/www.biblaridion.info\/blog\/immune-mythology\/embed\/#?secret=K5I7m0bWo8#?secret=9a9FNV3uVj\" data-secret=\"9a9FNV3uVj\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<blockquote class=\"twitter-tweet\" data-theme=\"dark\">\n<p dir=\"ltr\" lang=\"en\">The <a href=\"https:\/\/twitter.com\/hashtag\/stopcodon?src=hash&amp;ref_src=twsrc%5Etfw\">#stopcodon<\/a> issue is not going to magically go away.<\/p>\n<p>BioNtech included TWO stop codons.<br \/>\nModerna put in THREE.<\/p>\n<p>They knew about read-through. I can&#8217;t see any other explanation. <a href=\"https:\/\/twitter.com\/Kevin_McKernan?ref_src=twsrc%5Etfw\">@Kevin_McKernan<\/a> <a href=\"https:\/\/twitter.com\/Clucky92864053?ref_src=twsrc%5Etfw\">@Clucky92864053<\/a> <a href=\"https:\/\/t.co\/q1YmnObj7d\">pic.twitter.com\/q1YmnObj7d<\/a><\/p>\n<p>\u2014 Jikky Kjj \ud83d\udc2d (@JikkyKjj) <a href=\"https:\/\/twitter.com\/JikkyKjj\/status\/1488818619882811394?ref_src=twsrc%5Etfw\">February 2, 2022<\/a><\/p><\/blockquote>\n<p><script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script><\/p>\n<p>A cell replicates itself using its genomic intels. To do this, it reproduces what is available in DNA, until the stop codon (red) is met. The orange area is the most important part to get a perfect clone that does exactly what it is intended to do but, there is a way for the <strong>3\u2032-UTR<\/strong> (purple) to be included in the duplicated cell, which produces a cell that does something else. <strong>Something unknown.<\/strong><\/p>\n<p><strong>3\u2032-UTR<\/strong> is the <strong><a href=\"https:\/\/en.wikipedia.org\/wiki\/Three_prime_untranslated_region\">Three prime untranslated region <\/a><\/strong>. In molecular genetics, the three prime untranslated region (3\u2032-UTR) is the section of messenger RNA (mRNA) that immediately follows the translation termination codon. The 3\u2032-UTR often contains regulatory regions that post-transcriptionally influence gene expression.<\/p>\n<h2>Putting on the brakes<\/h2>\n<p>In order to prevent this happening they have put more than one stop condon in.<\/p>\n<p><a href=\"https:\/\/biology.stackexchange.com\/questions\/97482\/why-use-two-stop-uga-codons-instead-of-one-in-the-spike-protein-mrna-for-the-bio\"><strong>Why use two stop UGA codons instead of one in the spike protein mRNA for the BioNTech\/Pfizer SARS-CoV-2 vaccine?<\/strong><\/a><\/p>\n<p>&nbsp;<\/p>\n<h2>Read Through<\/h2>\n<p>People have valid concerns that &#8220;read through &#8221; will occur on the stop condons and it is known that in certain circumstances antibiotics can cause this. Here are some comments discussing the problem.<\/p>\n<p><a href=\"https:\/\/academic.oup.com\/nar\/article\/47\/2\/594\/5232438?login=false\"><strong>RefSeq curation and annotation of stop codon recoding in vertebrates<\/strong><\/a><\/p>\n<blockquote class=\"twitter-tweet\" data-theme=\"dark\">\n<p dir=\"ltr\" lang=\"en\">Whoah Clucky! That\u2019s a rabbit hole for me since aminoglycosides are our bread and butter in hospital.<br \/>\nG418=geneticin (similar to neomycin), not used clinically<\/p>\n<p>And then there\u2019s this<a href=\"https:\/\/t.co\/TevNB6aCwM\">https:\/\/t.co\/TevNB6aCwM<\/a><\/p>\n<p>\u2014 gutwat \u271d\ufe0f\ud83d\udcff\ud83d\udc2d\ud83c\udfe5\ud83d\udc8a\ud83e\udda0 (@gutwat1) <a href=\"https:\/\/twitter.com\/gutwat1\/status\/1488921409963368448?ref_src=twsrc%5Etfw\">February 2, 2022<\/a><\/p><\/blockquote>\n<p><script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script><\/p>\n<p>So it&#8217;s the alteration from uridine to pseudouridine allowing the codons to be read in the ribosome to instruct it to make the artificial S protein, but in the very act of that alteration, it screws up the &#8220;genetic compiler&#8221;, thereby misreading the code and translating the 3&#8217;UTR? There is a good chance that at least some (how many?) will readthrough causing the 3_UTR to be translated and attached to Spike Protein. And then that could cause Spike to mis-fold somehow (on this see McKernan). But NO testing was ever done to see what happens. They didn&#8217;t want to know.<\/p>\n<blockquote class=\"twitter-tweet\" data-theme=\"dark\">\n<p dir=\"ltr\" lang=\"en\">Pfizer uses UGA stop codon to optimise the mRNA, even though it&#8217;s the least good of the three, and then replaced the U with pseudouridine (to prevent mRNA degradation) but this could make the stop function even poorer. Double UGA to stop mistranslation.<a href=\"https:\/\/t.co\/igNpngx1jm\">https:\/\/t.co\/igNpngx1jm<\/a><\/p>\n<p>\u2014 Philip Soos (@PhilipSoos) <a href=\"https:\/\/twitter.com\/PhilipSoos\/status\/1489054993592193028?ref_src=twsrc%5Etfw\">February 3, 2022<\/a><\/p><\/blockquote>\n<p><script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script><\/p>\n<blockquote class=\"twitter-tweet\" data-theme=\"dark\">\n<p dir=\"ltr\" lang=\"en\">BNT162b2 from Pf(eye)zer. Well that uses the Groucho gene combined with mitochondrial 12s ribosome.<\/p>\n<p>You really don&#8217;t want your body to react to mitochondrial proteins. Honestly, you really don&#8217;t.<\/p>\n<p>Spike protein does enough damage in mitochondria<a href=\"https:\/\/t.co\/UrAup4Atdg\">https:\/\/t.co\/UrAup4Atdg<\/a> <a href=\"https:\/\/t.co\/wpvUxDwPDu\">pic.twitter.com\/wpvUxDwPDu<\/a><\/p>\n<p>\u2014 Jikky Kjj \ud83d\udc2d (@JikkyKjj) <a href=\"https:\/\/twitter.com\/JikkyKjj\/status\/1488818640808198144?ref_src=twsrc%5Etfw\">February 2, 2022<\/a><\/p><\/blockquote>\n<p><script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script><\/p>\n<p><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/labs\/pmc\/articles\/PMC7100133\/ \"><strong>If they already had their perfect 5&#8217;UTR in 2019 they wouldn&#8217;t have had to test 280,000 new ones, but what do I know eh?<\/strong><\/a><\/p>\n<h2>OAS and ADE<\/h2>\n<p><strong>Original Antigenic Sin and Antibody Dependent Enhancement.<\/strong><\/p>\n<p>&nbsp;<\/p>\n<blockquote class=\"twitter-tweet\" data-theme=\"dark\">\n<p dir=\"ltr\" lang=\"und\"><a href=\"https:\/\/t.co\/1P9aAyN3Aa\">https:\/\/t.co\/1P9aAyN3Aa<\/a><\/p>\n<p>\u2014 The Tishbite (@Tishbite_redux) <a href=\"https:\/\/twitter.com\/Tishbite_redux\/status\/1489477533196173315?ref_src=twsrc%5Etfw\">February 4, 2022<\/a><\/p><\/blockquote>\n<p><script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script><\/p>\n<p><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S2772613421000068\"><strong>The \u201coriginal antigenic sin\u201d and its relevance for SARS-CoV-2 (COVID-19) vaccination<\/strong><\/a><\/p>\n<blockquote class=\"twitter-tweet\" data-theme=\"dark\">\n<p dir=\"ltr\" lang=\"en\">The vaccinated are very fortunate that Omicron is a milder variant, because if it was reversed &#8211; greater virulence than Delta &#8211; the sickness and death it would cause would be catastrophic. This is what <a href=\"https:\/\/twitter.com\/GVDBossche?ref_src=twsrc%5Etfw\">@GVDBossche<\/a> has been warning about since the start. <a href=\"https:\/\/t.co\/DosoFaJ50D\">https:\/\/t.co\/DosoFaJ50D<\/a><\/p>\n<p>\u2014 Philip Soos (@PhilipSoos) <a href=\"https:\/\/twitter.com\/PhilipSoos\/status\/1489021028755853312?ref_src=twsrc%5Etfw\">February 2, 2022<\/a><\/p><\/blockquote>\n<p><script async src=\"https:\/\/platform.twitter.com\/widgets.js\" charset=\"utf-8\"><\/script><\/p>\n<h1>Conclusion<\/h1>\n<p>What would I know?\u00a0\u00a0 I am not a geneticist just a bucket chemist. However, I know enough to hear alarm bells sounding. Especially when pharma hides data.\u00a0 Especially when they have indemnity.<\/p>\n<h2 class=\"title style-scope ytd-video-primary-info-renderer\">Biohazard Alarm[WARNING: The sound is very LOUD] 49secs<\/h2>\n<p><iframe loading=\"lazy\" title=\"Biohazard Alarm[WARNING: The sound is very LOUD]\" width=\"614\" height=\"345\" src=\"https:\/\/www.youtube.com\/embed\/4VDqu7Oa9rs?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Stop Condons It is becoming clear that enormous risks have been taken with this experimental procedure which is the precursor platform to gene therapy.\u00a0 The Fourth Industrial Revolution is riding on this new technology and they have pushed ahead despite all the unknowns. In order to make it work they had to stabilize the mRNA [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[2191],"tags":[7017,3645,6975,6290,7016,4271,6284,7019,7020,6927,7021,7018],"class_list":["post-24704","post","type-post","status-publish","format-standard","hentry","category-vaccines","tag-3-utr","tag-ade","tag-methylate","tag-misfold","tag-oas","tag-prion","tag-pseudouridine","tag-read-through","tag-readthrough","tag-stop-condon","tag-three-prime-untranslated-region","tag-uridine"],"_links":{"self":[{"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/posts\/24704","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/comments?post=24704"}],"version-history":[{"count":11,"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/posts\/24704\/revisions"}],"predecessor-version":[{"id":24715,"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/posts\/24704\/revisions\/24715"}],"wp:attachment":[{"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/media?parent=24704"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/categories?post=24704"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.biblaridion.info\/blog\/wp-json\/wp\/v2\/tags?post=24704"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}