Tag: misfold

Stop Condons

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Stop Condons

It is becoming clear that enormous risks have been taken with this experimental procedure which is the precursor platform to gene therapy.  The Fourth Industrial Revolution is riding on this new technology and they have pushed ahead despite all the unknowns. In order to make it work they had to stabilize the mRNA otherwise it would be degraded by the body. They did this by methylating the uridine to pseudouridine.

However, this can cause other problems such as reading through “stop condons”.  If you have no idea what I am talking about I suggest you look at this previous article and specifically watch the video at the end with Dr Kevin McKernan and Dr. Jay Couey.

Sherlock Ohmes

 

A cell replicates itself using its genomic intels. To do this, it reproduces what is available in DNA, until the stop codon (red) is met. The orange area is the most important part to get a perfect clone that does exactly what it is intended to do but, there is a way for the 3′-UTR (purple) to be included in the duplicated cell, which produces a cell that does something else. Something unknown.

3′-UTR is the Three prime untranslated region . In molecular genetics, the three prime untranslated region (3′-UTR) is the section of messenger RNA (mRNA) that immediately follows the translation termination codon. The 3′-UTR often contains regulatory regions that post-transcriptionally influence gene expression.

Putting on the brakes

In order to prevent this happening they have put more than one stop condon in.

Why use two stop UGA codons instead of one in the spike protein mRNA for the BioNTech/Pfizer SARS-CoV-2 vaccine?

 

Read Through

People have valid concerns that “read through ” will occur on the stop condons and it is known that in certain circumstances antibiotics can cause this. Here are some comments discussing the problem.

RefSeq curation and annotation of stop codon recoding in vertebrates

So it’s the alteration from uridine to pseudouridine allowing the codons to be read in the ribosome to instruct it to make the artificial S protein, but in the very act of that alteration, it screws up the “genetic compiler”, thereby misreading the code and translating the 3’UTR? There is a good chance that at least some (how many?) will readthrough causing the 3_UTR to be translated and attached to Spike Protein. And then that could cause Spike to mis-fold somehow (on this see McKernan). But NO testing was ever done to see what happens. They didn’t want to know.

If they already had their perfect 5’UTR in 2019 they wouldn’t have had to test 280,000 new ones, but what do I know eh?

OAS and ADE

Original Antigenic Sin and Antibody Dependent Enhancement.

 

The “original antigenic sin” and its relevance for SARS-CoV-2 (COVID-19) vaccination

Conclusion

What would I know?   I am not a geneticist just a bucket chemist. However, I know enough to hear alarm bells sounding. Especially when pharma hides data.  Especially when they have indemnity.

Biohazard Alarm[WARNING: The sound is very LOUD] 49secs

mRNA stability

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mRNA stability

This post was inspired by the Couey vs Wilson debate which I am still transcribing and which can be found here:

Couey vs Wilson debate

The debate got rather heated and complex on the question of whether or not mRNA is an adjuvant or a self-adjuvant as their are studies that indicate that it could be used as an adjuvant.  Now, an adjuvant is a compound or chemical that irritates or triggers the immune system.

All vaccines have adjuvants and Dr Wilson (citing a scientific study) showed that mRNA could act as a self-adjuvant.    In other words there would be no need for the addition of an adjuvant.    The objection voiced by Jonathan was that mRNA is highly immunogenic. In other words it could trigger all sorts of autoimmune diseases and inflamation, especially if it was free floating in the blood.

In order to prevent this they need to do two things 1. encapsulate the mRNA in a Lipid Nano Particle (LNP)  and 2. stabilize the mRNA.  It turns out they do use PEG and polysorbate 80 as adjuvants.

 

PEG is a long chain polymer and its characteristics are different depending on the length of the polymer chain.  Polysorbate 80 is similar to PEG.   I have useed these substances as surfactants etc when making polymers. They have certain hydrophobic and hydrophilic qualities which can be used when mixing aqueous and oil phases and in micelle formation. The above paper lists MF59 adjuvant as an emulsion adjuvant composed of an oil phase (squalene 4.3%): and an aqueous phase (polysorbate 80:0.5%, sorbitan trioleate 0.5%). These vaccines do use an adjuvant, therefore Dr Wilson was incorrect.

Polyethylene glycol

A PEGylated lipid is used as an excipient in both the Moderna and Pfizer–BioNTech vaccines for SARS-CoV-2. Both RNA vaccines consist of messenger RNA, or mRNA, encased in a bubble of oily molecules called lipids. Proprietary lipid technology is used for each. In both vaccines, the bubbles are coated with a stabilizing molecule of polyethylene glycol.[medical citation needed] As of December 2020 there is some concern that PEG could trigger allergic reaction, and in fact allergic reactions are the driver for both the United Kingdom and Canadian regulators to issue an advisory, noting that: two “individuals in the U.K… were treated and have recovered” from anaphylactic shock. As of 18 December, the US CDC stated that in their jurisdiction six cases of “severe allergic reaction” had been recorded from more than 250,000 vaccinations, and of those six only one person had a “history of vaccination reactions”

Two things to mention here, firstly, many of the anaphylactic reactions are cause by the adjuvants like PEG and secondly, PEG and other polymers are being examined as delivery mechanisms for drugs because they cross the Blood Brain Barrier (BBB). If that is the case then they should also be able to deliver the spike protein across the BBB and evidence is emerging that happens.  That is not a good thing. There is a reason the brain is protected and segregated from the circulatory system and while it might be desirable to cross the BBB to treat diseases like dementia and Parkinson’s it comes with a risk.

So, as far as the debate is concerned Jonathan is correct. The vaccines do have an adjuvant and it is a LNP.  Note that the Wikipedia quote above uses the word excipient rather than adjuvant. An excipient is an inactive substance ….but is it really inactive? How can it be “inactive” when PEG antibodies are found and when some people suffer anaphylactic shock?

Polyethylene Glycol Immunogenicity: Theoretical, Clinical, and Practical Aspects of Anti-Polyethylene Glycol Antibodies

Pseudouridine

Wilson claimed that the self-adjuvant effect of mRNA was due to the pseudourodilatation of the mRNA. Pseudouridine is supposed to be more stable.

Ψ (pseudouridine) is also found in mRNAs which are the template for protein synthesis. Ψ residues in mRNA can affect the coding specificity of stop codons UAA, UGA, and UAG. In these stop codons both a U→Ψ modification and a U→C mutation both promote nonsense suppression.[6] In the SARS-CoV2 vaccine from BioNTech/Pfizer, also known as BNT162b2, Tozinameran or Comirnaty, all U's have been substituted with N1-methylpseudouridine,[7] a nucleoside related to Ψ that contains a methyl group added to N1 atom.

This is the tweet (second one Tishbite) that I sent out (well worth reading):

 

They’ve fiddled around with codon optimization to increase stability, but that translates into (1) enormous quantities produced of spike and (2) a different spike protein which could be even more pathogenic.

 

In case the above tweet disappears the link takes you here:

https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/

They are making people disappear off twitter (and FB)….this guy is good:

Conclusion

None of that fills me with confidence especially the fact that it can misfold.  That can lead to prions and neurodegeneration. Expect to see more diseases of the Central Nervous System,  dementia, Parkinson and CJD as well as more strokes, cancers and heart attacks. Don’t let them go anywhere near the kids with this.  We have been warning for more than a year and a half.  Is anyone listening?